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femtoCHROMO-AP Kit. No Antibodies. Enough For 4000cm2 Of Membrane

CODE: BE786-380

£210.00

Description
femto
CHROMO™ AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) washing buffer, femtoTBST™ Buffer.

In addition, kits containing AP labeled goat α-mouse and goat α-rabbit antibodies are also available. Please enquire for further details.

femtoCHROMO™ AP uses a modified BCIP (5-Bromo-4-Chloro-3'-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.


Figure: Detection with femtoCHROMO™. Normal (left) or tumor (right)
human lung lysates were resolved and transferred to a PVDF membrane.
Membrane was probed for actin and protein detected with goat anti-mouse
antibodies labeled with alkaline phosphatase (AP). Membrane was probed
with femtoCHROMO™-AP substrate.

Features

  • Detects >5ng
  • Ready-to-use
  • Single detection step
  • High signal to background ration
  • High reproducibility

Features not currently available

G-Biosciences

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