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femtoLUCENT™ PLUS-HRP

CODE: BE786-10

£263.00

Description
femtoLUCENT™ PLUS-HRP is a femtogram level sensitive chemiluminescence immunodetection system that allows users the option to detect even hard-to-detect low abundance proteins. This immunodetection system is based on an innovative formulation of luminol and 1, 2 dioxetane, a supersensitive detection reagent for HRP (horseradish peroxidase). The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. 

Figure. Biotin labeled casein was resolved by SDS-PAGE, transferred
to a PVDF membrane, and probed with a steptavidin-HRP conjugate.
The HRP was detected with femtoLUCENT™ PLUS.


This product is supplied in a kit format and contains non-animal protein blocking agent (NAP BLOCKER™) and  wash buffer (femto-TBST™).  The femtoLUCENT™ PLUS-HRP kit allow detection of femtogram levels (10-15) of antigens with low noise (signal/background) ratio. The kit reagents provided are sufficient for x25 mini blots or 1,500cm2 of PVDF or nitrocellulose membrane.

femtoLUCENT™ PLUS-HRP reagent kit is also available in a size suitable for x5, mini blots. femtoLUCENT™ PLUS-HRP substarte can alsop be purchased separately in 100, 200 and 500 ml volumes sifficient for 25, 50 and 125 blots respectively (each 4ml of working solution is suitable for 1 mini blot of 8 x 7.5cm).


Features

  • Reagents alone or kit formats
  • Intense light emission with low background and high signal
  • Supplied with a novel blocking agent to allow a rapid blocking step that produces a clear background
  • Economical: greater value compared to similar products

Features not currently available

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