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Human Cot-1 DNA, 500µg

CODE: BI-3001

£127.00

Description
Cot-1 DNA is commonly used to block non-speciofic hybridisation in micro-array screening. It is also used for In situ hybridisation, Comparative Genomic Hybridisation (CGH), and Genetic Analyzing.

COT I Human DNA is prepared from human male placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements. The COT I DNA fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements),are distributed ubiquitously throughout the genome.

Quality control:
- Average fragments size: 50-300 bp;
- A260/A280 ration:  about 1.78;
- Amount of genomic (non-repetitive DNA): less than 2%.

COT I DNA and the raw material is tested for the absence of HIV1,2 RNA, HCV RNA, HBV DNA

Recommendations for use:
For Southern blot hybridizations: add 50 μg of COT-1 human DNA (@ 10 μg/μL) to 50 μL of 20X SSC, 25 μL distilled water and 20 μL of a solution containing 0.1 M NaCl, 0.1 M Tris-HCl (pH 7.4).
0.01 M EDTA, and 1% SDS to the probe for each 25 to 500 ng of probe.

For in situ hybridizations: combine genomic probe with the proper amount of COT-1 human DNA such that the final concentration of COT-1 human DNA is 0.3 μg/μL for cosmid, plasmid, and lambda probes; or, at 1 μg/μL for Alu PCR probes. Ethanol precipitate and resuspend in a half-volume of 100% formamide. Add a half-volume of 20% dextran sulfate in 2X SSC (prewarmed to 75 degrees C) and mix well. Denature mix by heating to 75 °C for 5 min. Incubate at 37 °C for at least 5 and up to 15 min.


Specification

Concentration:
> 1,1 mg/ml

Storage Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.4

Transportation: Shipped on wet ice

Storage: -20°C for more than 12 months

Pack size: 500µg

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