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LB Broth (Lennox), 500g

CODE: WEB-1231


LB BROTH (LENNOX) is a nutritionally rich medium developed by Lennox for the growth and maintenance of pure culture of recombinant strains of E. coli. It is a rich growth medium that contains all the nutritional requirements for E.coli strains. In the formula, Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group. Sodium chloride supplies essential electrolytes for transport and osmotic balance. LB Broth (Lennox)contains ten times the sodium chloride level of Luria Broth (Miller’s Modification, Cat. WEB-1266) and a half of the level found in Luria Broth (Miller’s LB Broth, Cat. WEB-1551). This allows selecting the optimal salt concentration medium for a specific strain. Bacteria that contain plasmids tend to grow best in broth that has between 5 and 10 g of salt. For a faster growth, the medium can be supplemented with 0.1% glucose or 0.4% glycerol. Various cofactors may also need to be added to the broth if working with certain types of bacteriophages. For example, bacteriophage labmda requires an excess of magnesium in the broth to properly infect bacteria.

The cultivation in LB Broth allows the cells with an insert plasmid, to begin to express the genes on the transformed plasmid, including the antibiotic resistance gene. If the transformed E.coli are plated directly onto selective agar media (LB Agar containing antibiotic), fewer transformed colonies will appear per ml plated. Growing the transformed cells in LB broth will increase the number of transformed cells per ml. To select the bacteria with the plasmid, it is necessary to subcultivate an inoculum from LB Broth to a LB Agar plate with the antibiotic added

*For the test of Escherichia coli in molecular genetics studies

**For further information, including PREPARATION INSTRUCTIONS, please refer to Product Information Sheet.

Formula g/L

  • Tryptone (pancreatic digest of casein) 10g
  • Sodium Chloride 5g
  • Yeast Extract 5g

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