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Luria Broth (Miller's Modification), 500g

CODE: WEB-1266


LURIA BROTH (MILLER’S MODIFICATION) is based on LB Medium as described by Miller. Its modification consists of a minimal concentration of sodium chloride. It is suitable for the growth and maintenance of
E. coli strains used in molecular microbiology procedures. This is a nutritionally rich media designed for growth of pure cultures of recombinant strains. E. coli grows more rapidly because they provide the cells with amino acids, nucleotide precursors, vitamins and other metabolites that the microorganism would otherwise have to synthesize.

Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group. Sodium chloride supplies essential electrolytes for transport and osmotic balance. If desired, antibiotics can also be added. In Luria Broth (Miller’s Modification) sodium chloride level is ten times lower than in LB Broth(Lennox) (Cat. WEB-1231) and twenty times lower than in Luria (Miller’s LB Broth, Cat. WEB-1551).This allows selecting the optimal salt concentration medium for a specific strain.

*For molecular genetics studies using E.coli.

**For further information, including PREPARATION INSTRUCTIONS, please refer to Product Information Sheet.

Formula g/L

  • Tryptone (pancreatic digest of casein) 10g
  • Yeast Extract 5g
  • Sodium Chloride 0.5g

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