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Luria Agar (Miller's LB Agar), 500g

CODE: WEB-1552


LURIA AGAR (MILLER’S LB AGAR) is based on LB Medium as described by Miller for the growth and maintenance of
E. coli strains used in molecular microbiology procedures. It is nutritionally rich medium designed for growth of pure cultures of recombinant strains. E. coli grows faster because the Tryptone and Yeast extract supply essential growth factors such as nitrogen, carbon, sulphurs, minerals and vitamins, particularly B group and other metabolites that the microorganism would otherwise have to synthesize. Sodium Chloride supplies essential electrolytes for transport and osmotic balance.

If desired aseptically add 10 ml of sterile 20% glucose solution and mix thoroughly for a better growth. Bacteria that contain plasmids tend to grow best in broth that has between 5 and 10 g of salt. Various cofactors may also need to be added to the broth if working with certain types of bacteriophages. For example, bacteriophage lambda requires an excess of magnesium in the broth to properly infect bacteria. In Luria Agar (Miller’s LB Agar) sodium chloride level is two times higher than in LB Agar (Lennox, Cat. WEB-1083) and twenty times higher than in Luria Agar (Miller’s Modification, Cat. WEB-1308). This allows selecting the optimal salt concentration medium for a specific strain.

*For molecular genetics studies using E.coli.

**For further information, including PREPARATION INSTRUCTIONS, please refer to Product Information Sheet.

Formula g/L

  • Tryptone (pancreatic digest of casein) 10g
  • Sodium Chloride 10g
  • Yeast Extract 5g
  • Bacteriological Agar 15g

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