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SOB Medium, 500g

CODE: WEB-1541

£47.00

Description
SOB MEDIUM is a nutrient rich medium for the preparation and transformation of competent cells. The transformation requires perforation of the bacteria to allow the introduction of alien DNA inside the cell. In order to survive this process the competent cells need an isotonic rich medium.

Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group. Sodium chloride and Potassium chloride supplies essential electrolytes for transport and osmotic balance. Magnesium sulphate is a source of magnesium ions.

*For the cultivation of recombinant strains of E.coli.

**For further information, including PREPARATION INSTRUCTIONS, please refer to Product Information Sheet.

Formula g/L

  • Tryptone, 20g
  • Yeast Extract, 5g
  • Magnesium Sulphate, 0.96g
  • Sodium Chloride, 0.5g
  • Potassium Chloride, 0.186g

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